Expression of tdTomato and luciferase in a murine lung cancer alters the growth and immune microenvironment of the tumor
| العنوان: | Expression of tdTomato and luciferase in a murine lung cancer alters the growth and immune microenvironment of the tumor |
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| المؤلفون: | Xiaoxian Li, Ashwathi P. Menon, Mala Shanmugam, Suresh S. Ramalingam, Taofeek K. Owonikoko, Lei Huang, Ramireddy Bommireddy, Periasamy Selvaraj, Rohini N. Guin, Amanda Ruggieri, Changyong Wei, Luis E. Munoz |
| المصدر: | PLoS ONE, Vol 16, Iss 8, p e0254125 (2021) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Lung Neoplasms, Gene Expression, Biochemistry, Lung and Intrathoracic Tumors, Carcinoma, Lewis Lung, Mice, White Blood Cells, Spectrum Analysis Techniques, Genes, Reporter, Animal Cells, Tumor Microenvironment, Medicine and Health Sciences, Cytotoxic T cell, Luciferases, Multidisciplinary, biology, T Cells, Flow Cytometry, Enzymes, Oncology, Spectrophotometry, Medicine, Cytophotometry, Antibody, Cellular Types, Oxidoreductases, Luciferase, Research Article, Imaging Techniques, Immune Cells, Science, Immunology, Cytotoxic T cells, Research and Analysis Methods, Immune system, Cell Line, Tumor, Fluorescence Imaging, medicine, Animals, Molecular Biology Techniques, Molecular Biology, Tumor microenvironment, Blood Cells, Lewis lung carcinoma, Cancer, Cancers and Neoplasms, Biology and Life Sciences, Proteins, Cell Biology, medicine.disease, Luminescent Proteins, Tumor progression, Cancer cell, biology.protein, Cancer research, Enzymology, Cloning |
| الوصف: | Imaging techniques based on fluorescence and bioluminescence have been important tools in visualizing tumor progression and studying the effect of drugs and immunotherapies on tumor immune microenvironment in animal models of cancer. However, transgenic expression of foreign proteins may induce immune responses in immunocompetent syngeneic tumor transplant models and augment the efficacy of experimental drugs. In this study, we show that the growth rate of Lewis lung carcinoma (LL/2) tumors was reduced after transduction of tdTomato and luciferase (tdTomato/Luc) compared to the parental cell line. tdTomato/Luc expression by LL/2 cells altered the tumor microenvironment by increasing tumor-infiltrating lymphocytes (TILs) while inhibiting tumor-induced myeloid-derived suppressor cells (MDSCs). Interestingly, tdTomato/Luc expression did not alter the response of LL/2 tumors to anti-PD-1 and anti-CTLA-4 antibodies. These results suggest that the use of tdTomato/Luc-transduced cancer cells to conduct studies in immune competent mice may lead to cell-extrinsic tdTomato/Luc-induced alterations in tumor growth and tumor immune microenvironment that need to be taken into consideration when evaluating the efficacy of anti-cancer drugs and vaccines in immunocompetent animal models. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b37f45fe9dfb1f8371309e2b1d8845c7Test https://doaj.org/article/dc6e930ff2a44d15a2ac8e0dd7a119d8Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b37f45fe9dfb1f8371309e2b1d8845c7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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