المؤلفون: Fourdinier, Ophélie, Schepers, Eva, Metzinger-Le Meuth, Valérie, Glorieux, Griet, Liabeuf, Sophie, Verbeke, Francis, Vanholder, Raymond, Brigant, Benjamin, Pletinck, Anneleen, Diouf, Momar, Burtey, Stéphane, Choukroun, Gabriel, Massy, Ziad A., Metzinger, Laurent

المساهمون: INSERM U-1088, Université de Picardie Jules Verne (UPJV), CHU Amiens-Picardie, Ghent University Hospital, Laboratoire de Recherche Vasculaire Translationnelle (LVTS (UMR_S_1148 / U1148)), Université Paris 13 (UP13)-Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM), Mécanismes physiopathologiques et conséquences des calcifications vasculaires - UR UPJV 7517 (MP3CV), Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie, HEMATIM - Hématopoïèse et immunologie - UR UPJV 4666 (HEMATIM), Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research (C2VN), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre Hospitalier Universitaire Ambroise Paré (CHU Ambroise Paré)

المصدر: ISSN: 2045-2322.

مصطلحات موضوعية: [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology

الوصف: International audience ; Several microRNAs (miRNAs) have been linked to chronic kidney disease (CKD) mortality, cardiovascular (CV) complications and kidney disease progression. However, their association with clinical outcomes remains poorly evaluated. We used real-time qPCR to measure serum levels of miR-126 and miR-223 in a large cohort of 601 CKD patients (CKD stage G1 to G5 patients or on renal replacement therapy – CKD G5D) from Ghent University Hospital and 31 healthy controls. All-cause mortality and cardiovascular and renal events were registered as endpoints over a 6 year follow-up period. miR-126 levels were significantly lower from CKD stage G2 on, compared to controls. The serum levels of miR-223 were significantly lower from CKD stage G3B on. When considering overall mortality, patients with levels of either miR-126 or miR-223 below the median had a lower survival rate. Similar results were observed for CV and renal events. The observed link between the two miRNAs’ seric levels and mortality, cardiovascular events or renal events in CKD appears to depend on eGFR. However, this does not preclude their potential role in the pathophysiology of CKD. In conclusion, CKD is associated with a decrease in circulating miR-223 and miR-126 levels

العلاقة: hal-02625556; https://hal.inrae.fr/hal-02625556Test; https://hal.inrae.fr/hal-02625556/documentTest; https://hal.inrae.fr/hal-02625556/file/2019_Fourdinier_ScientificReports_1.pdfTest; PRODINRA: 476212; WOS: 000461151800061

الإتاحة: https://doi.org/10.1038/s41598-019-41101-8Test
https://hal.inrae.fr/hal-02625556Test
https://hal.inrae.fr/hal-02625556/documentTest
https://hal.inrae.fr/hal-02625556/file/2019_Fourdinier_ScientificReports_1.pdfTest

المؤلفون: Fourdinier, Ophélie, Schepers, Eva, Metzinger-Le Meuth, Valérie, Glorieux, Griet, Liabeuf, Sophie, Verbeke, Francis, Vanholder, Raymond, Brigant, Benjamin, Pletinck, Anneleen, Diouf, Momar, Burtey, Stéphane, Choukroun, Gabriel, Massy, Ziad A., Metzinger, Laurent

المساهمون: INSERM U-1088, Université de Picardie Jules Verne (UPJV), CHU Amiens-Picardie, Ghent University Hospital, Laboratoire de Recherche Vasculaire Translationnelle (LVTS (UMR_S_1148 / U1148)), Université Paris 13 (UP13)-Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM), Mécanismes physiopathologiques et conséquences des calcifications vasculaires - UR UPJV 7517 (MP3CV), Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie, HEMATIM - Hématopoïèse et immunologie - UR UPJV 4666 (HEMATIM), Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research (C2VN), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre Hospitalier Universitaire Ambroise Paré (CHU Ambroise Paré)

المصدر: ISSN: 2045-2322.

مصطلحات موضوعية: [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology

الوصف: International audience ; Several microRNAs (miRNAs) have been linked to chronic kidney disease (CKD) mortality, cardiovascular (CV) complications and kidney disease progression. However, their association with clinical outcomes remains poorly evaluated. We used real-time qPCR to measure serum levels of miR-126 and miR-223 in a large cohort of 601 CKD patients (CKD stage G1 to G5 patients or on renal replacement therapy – CKD G5D) from Ghent University Hospital and 31 healthy controls. All-cause mortality and cardiovascular and renal events were registered as endpoints over a 6 year follow-up period. miR-126 levels were significantly lower from CKD stage G2 on, compared to controls. The serum levels of miR-223 were significantly lower from CKD stage G3B on. When considering overall mortality, patients with levels of either miR-126 or miR-223 below the median had a lower survival rate. Similar results were observed for CV and renal events. The observed link between the two miRNAs’ seric levels and mortality, cardiovascular events or renal events in CKD appears to depend on eGFR. However, this does not preclude their potential role in the pathophysiology of CKD. In conclusion, CKD is associated with a decrease in circulating miR-223 and miR-126 levels

العلاقة: hal-02625556; https://hal.inrae.fr/hal-02625556Test; https://hal.inrae.fr/hal-02625556/documentTest; https://hal.inrae.fr/hal-02625556/file/2019_Fourdinier_ScientificReports_1.pdfTest; PRODINRA: 476212; WOS: 000461151800061

الإتاحة: https://doi.org/10.1038/s41598-019-41101-8Test
https://hal.inrae.fr/hal-02625556Test
https://hal.inrae.fr/hal-02625556/documentTest
https://hal.inrae.fr/hal-02625556/file/2019_Fourdinier_ScientificReports_1.pdfTest

المؤلفون: Fourdinier, Ophélie, Schepers, Eva, Metzinger-Le Meuth, Valérie, Glorieux, Griet, Liabeuf, Sophie, Verbeke, Francis, Vanholder, Raymond, Brigant, Benjamin, Pletinck, Anneleen, Diouf, Momar, Burtey, Stéphane, Choukroun, Gabriel, Massy, Ziad A., Metzinger, Laurent

المساهمون: INSERM U-1088, Université de Picardie Jules Verne (UPJV), CHU Amiens-Picardie, Ghent University Hospital, Laboratoire de Recherche Vasculaire Translationnelle (LVTS (UMR_S_1148 / U1148)), Université Paris 13 (UP13)-Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM), Mécanismes physiopathologiques et conséquences des calcifications vasculaires - UR UPJV 7517 (MP3CV), Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie, HEMATIM - Hématopoïèse et immunologie - UR UPJV 4666 (HEMATIM), Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research (C2VN), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre Hospitalier Universitaire Ambroise Paré (CHU Ambroise Paré)

المصدر: ISSN: 2045-2322.

مصطلحات موضوعية: [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology

الوصف: International audience ; Several microRNAs (miRNAs) have been linked to chronic kidney disease (CKD) mortality, cardiovascular (CV) complications and kidney disease progression. However, their association with clinical outcomes remains poorly evaluated. We used real-time qPCR to measure serum levels of miR-126 and miR-223 in a large cohort of 601 CKD patients (CKD stage G1 to G5 patients or on renal replacement therapy – CKD G5D) from Ghent University Hospital and 31 healthy controls. All-cause mortality and cardiovascular and renal events were registered as endpoints over a 6 year follow-up period. miR-126 levels were significantly lower from CKD stage G2 on, compared to controls. The serum levels of miR-223 were significantly lower from CKD stage G3B on. When considering overall mortality, patients with levels of either miR-126 or miR-223 below the median had a lower survival rate. Similar results were observed for CV and renal events. The observed link between the two miRNAs’ seric levels and mortality, cardiovascular events or renal events in CKD appears to depend on eGFR. However, this does not preclude their potential role in the pathophysiology of CKD. In conclusion, CKD is associated with a decrease in circulating miR-223 and miR-126 levels

العلاقة: hal-02625556; https://hal.inrae.fr/hal-02625556Test; https://hal.inrae.fr/hal-02625556/documentTest; https://hal.inrae.fr/hal-02625556/file/2019_Fourdinier_ScientificReports_1.pdfTest; PRODINRA: 476212; WOS: 000461151800061

الإتاحة: https://doi.org/10.1038/s41598-019-41101-8Test
https://hal.inrae.fr/hal-02625556Test
https://hal.inrae.fr/hal-02625556/documentTest
https://hal.inrae.fr/hal-02625556/file/2019_Fourdinier_ScientificReports_1.pdfTest

المؤلفون: Fourdinier, Ophélie, Schepers, Eva, Metzinger-Le Meuth, Valérie, Glorieux, Griet, Liabeuf, Sophie, Verbeke, Francis, Vanholder, Raymond, Brigant, Benjamin, Pletinck, Anneleen, Diouf, Momar, Burtey, Stéphane, Choukroun, Gabriel, Massy, Ziad A., Metzinger, Laurent

المساهمون: INSERM U-1088, Université de Picardie Jules Verne (UPJV), CHU Amiens-Picardie, Ghent University Hospital, Laboratoire de Recherche Vasculaire Translationnelle (LVTS (UMR_S_1148 / U1148)), Université Paris 13 (UP13)-Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM), Mécanismes physiopathologiques et conséquences des calcifications vasculaires - UR UPJV 7517 (MP3CV), Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie, HEMATIM - Hématopoïèse et immunologie - UR UPJV 4666 (HEMATIM), Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research (C2VN), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre Hospitalier Universitaire Ambroise Paré (CHU Ambroise Paré)

المصدر: ISSN: 2045-2322.

مصطلحات موضوعية: [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology

الوصف: International audience ; Several microRNAs (miRNAs) have been linked to chronic kidney disease (CKD) mortality, cardiovascular (CV) complications and kidney disease progression. However, their association with clinical outcomes remains poorly evaluated. We used real-time qPCR to measure serum levels of miR-126 and miR-223 in a large cohort of 601 CKD patients (CKD stage G1 to G5 patients or on renal replacement therapy – CKD G5D) from Ghent University Hospital and 31 healthy controls. All-cause mortality and cardiovascular and renal events were registered as endpoints over a 6 year follow-up period. miR-126 levels were significantly lower from CKD stage G2 on, compared to controls. The serum levels of miR-223 were significantly lower from CKD stage G3B on. When considering overall mortality, patients with levels of either miR-126 or miR-223 below the median had a lower survival rate. Similar results were observed for CV and renal events. The observed link between the two miRNAs’ seric levels and mortality, cardiovascular events or renal events in CKD appears to depend on eGFR. However, this does not preclude their potential role in the pathophysiology of CKD. In conclusion, CKD is associated with a decrease in circulating miR-223 and miR-126 levels

العلاقة: hal-02625556; https://hal.inrae.fr/hal-02625556Test; https://hal.inrae.fr/hal-02625556/documentTest; https://hal.inrae.fr/hal-02625556/file/2019_Fourdinier_ScientificReports_1.pdfTest; PRODINRA: 476212; WOS: 000461151800061

الإتاحة: https://doi.org/10.1038/s41598-019-41101-8Test
https://hal.inrae.fr/hal-02625556Test
https://hal.inrae.fr/hal-02625556/documentTest
https://hal.inrae.fr/hal-02625556/file/2019_Fourdinier_ScientificReports_1.pdfTest

المؤلفون: Fourdinier, Ophélie, Schepers, Eva, Metzinger-Le Meuth, Valérie, Glorieux, Griet, Liabeuf, Sophie, Verbeke, Francis, Vanholder, Raymond, Brigant, Benjamin, Pletinck, Anneleen, Diouf, Momar, Burtey, Stéphane, Choukroun, Gabriel, Massy, Ziad A., Metzinger, Laurent

المساهمون: INSERM U-1088, Université de Picardie Jules Verne (UPJV), CHU Amiens-Picardie, Ghent University Hospital, Laboratoire de Recherche Vasculaire Translationnelle (LVTS (UMR_S_1148 / U1148)), Université Paris 13 (UP13)-Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM), Mécanismes physiopathologiques et conséquences des calcifications vasculaires - UR UPJV 7517 (MP3CV), Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie, HEMATIM - Hématopoïèse et immunologie - UR UPJV 4666 (HEMATIM), Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research (C2VN), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre Hospitalier Universitaire Ambroise Paré (CHU Ambroise Paré)

المصدر: ISSN: 2045-2322.

مصطلحات موضوعية: [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology

الوصف: International audience ; Several microRNAs (miRNAs) have been linked to chronic kidney disease (CKD) mortality, cardiovascular (CV) complications and kidney disease progression. However, their association with clinical outcomes remains poorly evaluated. We used real-time qPCR to measure serum levels of miR-126 and miR-223 in a large cohort of 601 CKD patients (CKD stage G1 to G5 patients or on renal replacement therapy – CKD G5D) from Ghent University Hospital and 31 healthy controls. All-cause mortality and cardiovascular and renal events were registered as endpoints over a 6 year follow-up period. miR-126 levels were significantly lower from CKD stage G2 on, compared to controls. The serum levels of miR-223 were significantly lower from CKD stage G3B on. When considering overall mortality, patients with levels of either miR-126 or miR-223 below the median had a lower survival rate. Similar results were observed for CV and renal events. The observed link between the two miRNAs’ seric levels and mortality, cardiovascular events or renal events in CKD appears to depend on eGFR. However, this does not preclude their potential role in the pathophysiology of CKD. In conclusion, CKD is associated with a decrease in circulating miR-223 and miR-126 levels

العلاقة: hal-02625556; https://hal.inrae.fr/hal-02625556Test; https://hal.inrae.fr/hal-02625556/documentTest; https://hal.inrae.fr/hal-02625556/file/2019_Fourdinier_ScientificReports_1.pdfTest; PRODINRA: 476212; WOS: 000461151800061

الإتاحة: https://doi.org/10.1038/s41598-019-41101-8Test
https://hal.inrae.fr/hal-02625556Test
https://hal.inrae.fr/hal-02625556/documentTest
https://hal.inrae.fr/hal-02625556/file/2019_Fourdinier_ScientificReports_1.pdfTest

المؤلفون: Fourdinier, Ophélie, Schepers, Eva, Metzinger-Le Meuth, Valérie, Glorieux, Griet, Liabeuf, Sophie, Verbeke, Francis, Vanholder, Raymond, Brigant, Benjamin, Pletinck, Anneleen, Diouf, Momar, Burtey, Stéphane, Choukroun, Gabriel, Massy, Ziad A., Metzinger, Laurent

المساهمون: INSERM U-1088, Université de Picardie Jules Verne (UPJV), CHU Amiens-Picardie, Ghent University Hospital, Laboratoire de Recherche Vasculaire Translationnelle (LVTS (UMR_S_1148 / U1148)), Université Paris Diderot - Paris 7 (UPD7)-Université Paris 13 (UP13)-Institut National de la Santé et de la Recherche Médicale (INSERM), HEMATIM - Hématopoïèse et immunologie - UR UPJV 4666 (HEMATIM), Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research (C2VN), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre Hospitalier Universitaire Ambroise Paré (CHU Ambroise Paré)

المصدر: ISSN: 2045-2322.

مصطلحات موضوعية: [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology

الوصف: International audience ; Several microRNAs (miRNAs) have been linked to chronic kidney disease (CKD) mortality, cardiovascular (CV) complications and kidney disease progression. However, their association with clinical outcomes remains poorly evaluated. We used real-time qPCR to measure serum levels of miR-126 and miR-223 in a large cohort of 601 CKD patients (CKD stage G1 to G5 patients or on renal replacement therapy – CKD G5D) from Ghent University Hospital and 31 healthy controls. All-cause mortality and cardiovascular and renal events were registered as endpoints over a 6 year follow-up period. miR-126 levels were significantly lower from CKD stage G2 on, compared to controls. The serum levels of miR-223 were significantly lower from CKD stage G3B on. When considering overall mortality, patients with levels of either miR-126 or miR-223 below the median had a lower survival rate. Similar results were observed for CV and renal events. The observed link between the two miRNAs’ seric levels and mortality, cardiovascular events or renal events in CKD appears to depend on eGFR. However, this does not preclude their potential role in the pathophysiology of CKD. In conclusion, CKD is associated with a decrease in circulating miR-223 and miR-126 levels

العلاقة: hal-02625556; https://hal.inrae.fr/hal-02625556Test; https://hal.inrae.fr/hal-02625556/documentTest; https://hal.inrae.fr/hal-02625556/file/2019_Fourdinier_ScientificReports_1.pdfTest; PRODINRA: 476212; WOS: 000461151800061

الإتاحة: https://doi.org/10.1038/s41598-019-41101-8Test
https://hal.inrae.fr/hal-02625556Test
https://hal.inrae.fr/hal-02625556/documentTest
https://hal.inrae.fr/hal-02625556/file/2019_Fourdinier_ScientificReports_1.pdfTest

المؤلفون: Fourdinier, Ophélie, Schepers, Eva, Metzinger-Le Meuth, Valérie, Glorieux, Griet, Liabeuf, Sophie, Verbeke, Francis, Vanholder, Raymond, Brigant, Benjamin, Pletinck, Anneleen, Diouf, Momar, Burtey, Stéphane, Choukroun, Gabriel, Massy, Ziad A., Metzinger, Laurent, Argiles, Angel, Beige, Joachim, Brunet, Philippe, Cohen, Gerald, Deif, Omar Abou, Evenepoel, Pieter, Fliser, Danilo, Fridolin, Ivo, Gmerek, Andreas, Jankowski, Joachim, Jankowski, Vera, Masereeuw, Roos, Mischak, Harald, Ortiz, Alberto, Perna, Alessandra, Rodriguez-Portillo, Juan Mariano, Schanstra, Joost, Spasovski, Goce, Stamatialis, Dimitrios, Steppan, Sonja, Storr, Markus, Stegmayr, Bernd G., Stenvinkel, Peter, Thornalley, Paul J., Wiecek, Andrej

المساهمون: Fourdinier, Ophélie, Schepers, Eva, Metzinger-Le Meuth, Valérie, Glorieux, Griet, Liabeuf, Sophie, Verbeke, Franci, Vanholder, Raymond, Brigant, Benjamin, Pletinck, Anneleen, Diouf, Momar, Burtey, Stéphane, Choukroun, Gabriel, Massy, Ziad A., Metzinger, Laurent, Argiles, Angel, Beige, Joachim, Brunet, Philippe, Cohen, Gerald, Deif, Omar Abou, Evenepoel, Pieter, Fliser, Danilo, Fridolin, Ivo, Gmerek, Andrea, Jankowski, Joachim, Jankowski, Vera, Masereeuw, Roo, Mischak, Harald, Ortiz, Alberto, Perna, Alessandra, Rodriguez-Portillo, Juan Mariano, Schanstra, Joost, Spasovski, Goce, Stamatialis, Dimitrio, Steppan, Sonja, Storr, Marku, Stegmayr, Bernd G., Stenvinkel, Peter, Thornalley, Paul J., Wiecek, Andrej

مصطلحات موضوعية: Multidisciplinary

الوصف: Several microRNAs (miRNAs) have been linked to chronic kidney disease (CKD) mortality, cardiovascular (CV) complications and kidney disease progression. However, their association with clinical outcomes remains poorly evaluated. We used real-time qPCR to measure serum levels of miR-126 and miR-223 in a large cohort of 601 CKD patients (CKD stage G1 to G5 patients or on renal replacement therapy -CKD G5D) from Ghent University Hospital and 31 healthy controls. All-cause mortality and cardiovascular and renal events were registered as endpoints over a 6 year follow-up period. miR-126 levels were significantly lower from CKD stage G2 on, compared to controls. The serum levels of miR-223 were significantly lower from CKD stage G3B on. When considering overall mortality, patients with levels of either miR-126 or miR-223 below the median had a lower survival rate. Similar results were observed for CV and renal events. The observed link between the two miRNAs' seric levels and mortality, cardiovascular events or renal events in CKD appears to depend on eGFR. However, this does not preclude their potential role in the pathophysiology of CKD. In conclusion, CKD is associated with a decrease in circulating miR-223 and miR-126 levels.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/30872798; info:eu-repo/semantics/altIdentifier/wos/WOS:000461151800061; volume:9; issue:1; firstpage:4477; journal:SCIENTIFIC REPORTS; http://hdl.handle.net/11591/405261Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85062959371; www.nature.com/srep/index.html

الإتاحة: https://doi.org/10.1038/s41598-019-41101-8Test
http://hdl.handle.net/11591/405261Test

المؤلفون: Fourdinier, Ophélie, Schepers, Eva, Metzinger-Le Meuth, Valérie, Glorieux, Griet, Liabeuf, Sophie, Verbeke, Francis, Vanholder, Raymond, Brigant, Benjamin, Pletinck, Anneleen, Diouf, Momar, Burtey, Stéphane, Choukroun, Gabriel, Massy, Ziad A., Metzinger, Laurent, Thornalley, Paul J.

العلاقة: Fourdinier, Ophélie, Schepers, Eva, Metzinger-Le Meuth, Valérie, Glorieux, Griet, Liabeuf, Sophie, Verbeke, Francis, Vanholder, Raymond, Brigant, Benjamin, Pletinck, Anneleen, Diouf, Momar, Burtey, Stéphane, Choukroun, Gabriel, Massy, Ziad A., Metzinger, Laurent and Thornalley, Paul J. (2019) Serum levels of miR-126 and miR-223 and outcomes in chronic kidney disease patients. Scientific Reports, 9 (1). doi:10.1038/s41598-019-41101-8

الإتاحة: https://doi.org/10.1038/s41598-019-41101-8Test
http://wrap.warwick.ac.uk/119969Test/

المؤلفون: Gondouin, Bertrand, Cerini, Claire, Dou, Laetitia, Sallée, Marion, Duval-Sabatier, Ariane, Pletinck, Anneleen, Calaf, Raymond, Lacroix, Romaric, Jourde-Chiche, Noemie, Poitevin, Stéphane, Arnaud, Laurent, Vanholder, Raymond, Brunet, Philippe, Dignat-George, Francoise, Burtey, Stéphane

المساهمون: Vascular research center of Marseille (VRCM), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Hôpital de la Conception CHU - APHM (LA CONCEPTION), Nephrology Section Ghent, Ghent University Hospital

المصدر: ISSN: 0085-2538.

مصطلحات موضوعية: [SDV.MHEP.UN]Life Sciences [q-bio]/Human health and pathology/Urology and Nephrology, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system

الوصف: International audience ; In chronic kidney disease (CKD), uremic solutes accumulate in blood and tissues. These compounds probably contribute to the marked increase in cardiovascular risk during the progression of CKD. The uremic solutes indoxyl sulfate and indole-3-acetic acid (IAA) are particularly deleterious for endothelial cells. Here we performed microarray and comparative PCR analyses to identify genes in endothelial cells targeted by these two uremic solutes. We found an increase in endothelial expression of tissue factor in response to indoxyl sulfate and IAA and upregulation of eight genes regulated by the transcription factor aryl hydrocarbon receptor (AHR). The suggestion by microarray analysis of an involvement of AHR in tissue factor production was confirmed by siRNA inhibition and the indirect AHR inhibitor geldanamycin. These observations were extended to peripheral blood mononuclear cells. Tissue factor expression and activity were also increased by AHR agonist dioxin. Finally, we measured circulating tissue factor concentration and activity in healthy control subjects and in patients with CKD (stages 3-5d), and found that each was elevated in patients with CKD. Circulating tissue factor levels were positively correlated with plasma indoxyl sulfate and IAA. Thus, indolic uremic solutes increase tissue factor production in endothelial and peripheral blood mononuclear cells by AHR activation, evoking a `dioxin-like' effect. This newly described mechanism of uremic solute toxicity may help understand the high cardiovascular risk of CKD patients.

العلاقة: hal-01610430; https://hal.science/hal-01610430Test

الإتاحة: https://doi.org/10.1038/ki.2013.133Test
https://hal.science/hal-01610430Test

المؤلفون: Gondouin, Bertrand, Cerini, Claire, Dou, Laetitia, Sallée, Marion, Duval-Sabatier, Ariane, Pletinck, Anneleen, Calaf, Raymond, Lacroix, Romaric, Jourde-Chiche, Noemie, Poitevin, Stéphane, Arnaud, Laurent, Vanholder, Raymond, BRUNET, Philippe, Dignat-George, Francoise, Burtey, Stéphane

المساهمون: Vascular research center of Marseille (VRCM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Aix Marseille Université (AMU), Hôpital de la Conception CHU - APHM (LA CONCEPTION), Nephrology Section Ghent, Ghent University Hospital

المصدر: ISSN: 0085-2538.

مصطلحات موضوعية: [SDV.MHEP.UN]Life Sciences [q-bio]/Human health and pathology/Urology and Nephrology, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system

الوصف: International audience ; In chronic kidney disease (CKD), uremic solutes accumulate in blood and tissues. These compounds probably contribute to the marked increase in cardiovascular risk during the progression of CKD. The uremic solutes indoxyl sulfate and indole-3-acetic acid (IAA) are particularly deleterious for endothelial cells. Here we performed microarray and comparative PCR analyses to identify genes in endothelial cells targeted by these two uremic solutes. We found an increase in endothelial expression of tissue factor in response to indoxyl sulfate and IAA and upregulation of eight genes regulated by the transcription factor aryl hydrocarbon receptor (AHR). The suggestion by microarray analysis of an involvement of AHR in tissue factor production was confirmed by siRNA inhibition and the indirect AHR inhibitor geldanamycin. These observations were extended to peripheral blood mononuclear cells. Tissue factor expression and activity were also increased by AHR agonist dioxin. Finally, we measured circulating tissue factor concentration and activity in healthy control subjects and in patients with CKD (stages 3-5d), and found that each was elevated in patients with CKD. Circulating tissue factor levels were positively correlated with plasma indoxyl sulfate and IAA. Thus, indolic uremic solutes increase tissue factor production in endothelial and peripheral blood mononuclear cells by AHR activation, evoking a `dioxin-like' effect. This newly described mechanism of uremic solute toxicity may help understand the high cardiovascular risk of CKD patients.

العلاقة: hal-01610430; https://hal.archives-ouvertes.fr/hal-01610430Test

الإتاحة: https://doi.org/10.1038/ki.2013.133Test
https://hal.archives-ouvertes.fr/hal-01610430Test