Flow Cytometric Analysis of Myeloid Cells in Human Blood, Bronchoalveolar Lavage, and Lung Tissues
| العنوان: | Flow Cytometric Analysis of Myeloid Cells in Human Blood, Bronchoalveolar Lavage, and Lung Tissues |
|---|---|
| المؤلفون: | Robert M. Tighe, John W. Hollingsworth, Paul W. Noble, Yen-Rei A. Yu, Andrew J. Ghio, Danielle F. Hotten, Yuryi Malakhau, Michael D. Gunn, Monica Kraft, Ellen Volker |
| المصدر: | American Journal of Respiratory Cell and Molecular Biology. 54:13-24 |
| بيانات النشر: | American Thoracic Society, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Adult, Male, 0301 basic medicine, Pulmonary and Respiratory Medicine, Pathology, medicine.medical_specialty, Adolescent, Sialic Acid Binding Ig-like Lectin 1, CD14, Clinical Biochemistry, CD11c, Biology, Immunophenotyping, Flow cytometry, Young Adult, 03 medical and health sciences, 0302 clinical medicine, Macrophages, Alveolar, medicine, Humans, Myeloid Cells, Lung, Molecular Biology, Original Research, Microscopy, Confocal, Cluster of differentiation, medicine.diagnostic_test, Monocyte, Interstitial lung disease, Cell Biology, respiratory system, Flow Cytometry, medicine.disease, respiratory tract diseases, 030104 developmental biology, medicine.anatomical_structure, Bronchoalveolar lavage, Female, Bronchoalveolar Lavage Fluid, Biomarkers, 030215 immunology |
| الوصف: | Clear identification of specific cell populations by flow cytometry is important to understand functional roles. A well-defined flow cytometry panel for myeloid cells in human bronchoalveolar lavage (BAL) and lung tissue is currently lacking. The objective of this study was to develop a flow cytometry-based panel for human BAL and lung tissue. We obtained and performed flow cytometry/sorting on human BAL cells and lung tissue. Confocal images were obtained from lung tissue using antibodies for cluster of differentiation (CD)206, CD169, and E cadherin. We defined a multicolor flow panel for human BAL and lung tissue that identifies major leukocyte populations. These include macrophage (CD206(+)) subsets and other CD206(-) leukocytes. The CD206(-) cells include: (1) three monocyte (CD14(+)) subsets, (2) CD11c(+) dendritic cells (CD14(-), CD11c(+), HLA-DR(+)), (3) plasmacytoid dendritic cells (CD14(-), CD11c(-), HLA-DR(+), CD123(+)), and (4) other granulocytes (neutrophils, mast cells, eosinophils, and basophils). Using this panel on human lung tissue, we defined two populations of pulmonary macrophages: CD169(+) and CD169(-) macrophages. In lung tissue, CD169(-) macrophages were a prominent cell type. Using confocal microscopy, CD169(+) macrophages were located in the alveolar space/airway, defining them as alveolar macrophages. In contrast, CD169(-) macrophages were associated with airway/alveolar epithelium, consistent with interstitial-associated macrophages. We defined a flow cytometry panel in human BAL and lung tissue that allows identification of multiple immune cell types and delineates alveolar from interstitial-associated macrophages. This study has important implications for defining myeloid cells in human lung samples. |
| تدمد: | 1535-4989 1044-1549 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c73ba08784e9669c31aafe8d8c05b8feTest https://doi.org/10.1165/rcmb.2015-0146ocTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c73ba08784e9669c31aafe8d8c05b8fe |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15354989 10441549 |
|---|