التفاصيل البيبلوغرافية
| العنوان: |
Spore Coat Architecture of Clostridium novyi NT Spores |
| المؤلفون: |
Plomp, Marco, McCaffery, J. Michael, Cheong, Ian, Huang, Xin, Bettegowda, Chetan, Kinzler, Kenneth W., Zhou, Shibin, Vogelstein, Bert, Malkin, Alexander J. |
| المصدر: |
Journal of Bacteriology ; volume 189, issue 17, page 6457-6468 ; ISSN 0021-9193 1098-5530 |
| بيانات النشر: |
American Society for Microbiology |
| سنة النشر: |
2007 |
| الوصف: |
Spores of the anaerobic bacterium Clostridium novyi NT are able to germinate in and destroy hypoxic regions of tumors in experimental animals. Future progress in this area will benefit from a better understanding of the germination and outgrowth processes that are essential for the tumorilytic properties of these spores. Toward this end, we have used both transmission electron microscopy and atomic force microscopy to determine the structure of both dormant and germinating spores. We found that the spores are surrounded by an amorphous layer intertwined with honeycomb parasporal layers. Moreover, the spore coat layers had apparently self-assembled, and this assembly was likely to be governed by crystal growth principles. During germination and outgrowth, the honeycomb layers, as well as the underlying spore coat and undercoat layers, sequentially dissolved until the vegetative cell was released. In addition to their implications for understanding the biology of C. novyi NT, these studies document the presence of proteinaceous growth spirals in a biological organism. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1128/jb.00757-07 |
| DOI: |
10.1128/JB.00757-07 |
| الإتاحة: |
https://doi.org/10.1128/jb.00757-07Test |
| حقوق: |
https://journals.asm.org/non-commercial-tdm-licenseTest |
| رقم الانضمام: |
edsbas.9532386 |
| قاعدة البيانات: |
BASE |
