Synaptotagmin IX, a possible linker between the perinuclear endocytic recycling compartment and the microtubules
| العنوان: | Synaptotagmin IX, a possible linker between the perinuclear endocytic recycling compartment and the microtubules |
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| المؤلفون: | Yael Haberman, Mitsunori Fukuda, Elena Grimberg, Ronit Sagi-Eisenberg |
| المصدر: | Journal of Cell Science. 116:4307-4318 |
| بيانات النشر: | The Company of Biologists, 2003. |
| سنة النشر: | 2003 |
| مصطلحات موضوعية: | Endosome, Recombinant Fusion Proteins, Endocytic recycling, Nerve Tissue Proteins, Endosomes, Biology, Microtubules, Synaptotagmin 1, Synaptotagmins, symbols.namesake, Microtubule, Chlorocebus aethiops, Animals, Mast Cells, Cells, Cultured, Membrane Glycoproteins, Calcium-Binding Proteins, Cell Membrane, Transferrin, Cell Biology, Golgi apparatus, Fusion protein, Endocytosis, Cell Compartmentation, Protein Structure, Tertiary, Rats, Cell biology, Tubulin, Microscopy, Fluorescence, Mesothelin, COS Cells, biology.protein, symbols, Rab, Microtubule-Organizing Center, Subcellular Fractions |
| الوصف: | The pericentriolar endocytic recycling compartment (ERC) is involved in receptor and lipid recycling as well as in the delivery of internalized cargo from early endosomes to the trans Golgi network (TGN). We show that synaptotagmin (Syt) IX, a member of the Syt family of proteins, localizes to the ERC and is required for export from the ERC to the cell surface. We demonstrate that rat basophilic leukemia (RBL-2H3) mast cells endogenously express Syt IX mRNA and protein. Localization studies employing fractionation on linear sucrose gradients combined with confocal microscopy by indirect immunofluorescence or stable expression of a Syt IX-green fluorescent fusion protein demonstrate that Syt IX colocalizes with internalized transferrin (Tfn) and with Rab 11 at the perinuclear ERC. Syt IX also colocalizes with tubulin at the microtubules organizing center (MTOC) and remains associated with tubulin clusters formed in taxol-treated cells. Moreover, Syt IX coimmunoprecipitates with tubulin from intact RBL cells, and chimeric fusion proteins comprising either the C2A or the C2B domain of Syt IX are able to pull down tubulin from RBL cell lysates. To study the functional role of Syt IX, we have stably transfected RBL cells with Syt IX sense or antisense cDNA and monitored the routes of Tfn internalization and recycling in cells that overexpress (RBL-Syt IX+) or display substantially reduced ( |
| تدمد: | 1477-9137 0021-9533 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9b330f76f1b4db77fa1d69fe5381a067Test https://doi.org/10.1242/jcs.00719Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....9b330f76f1b4db77fa1d69fe5381a067 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14779137 00219533 |
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