Nicotinic acid modulates Legionella pneumophila gene expression and induces virulence traits
| العنوان: | Nicotinic acid modulates Legionella pneumophila gene expression and induces virulence traits |
|---|---|
| المؤلفون: | Kaoru Harada, Rachel L. Edwards, Michele S. Swanson, Matthieu Jules, Andrew Bryan, Carmen Buchrieser |
| المساهمون: | Cellular and Molecular Biology Program, University of Michigan [Ann Arbor], University of Michigan System-University of Michigan System, Department of Microbiology and Immunology, University of Michigan Medical School, Biologie des Bactéries intracellulaires - Biology of Intracellular Bacteria, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Biologie des bactéries intracellulaires, Research in the Swanson lab was supported by a Rackham Graduate Student Research Award and NIH grant RO1 AI044212. Research in the Buchrieser lab was supported by the Institut Pasteur, the Centre National de la Recherche Scientifique (CNRS), and the Fondation pour la Recherche Médicale (FRM)., Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | Infection and Immunity Infection and Immunity, American Society for Microbiology, 2013, 81 (3), pp.945-55. ⟨10.1128/IAI.00999-12⟩ Infection and Immunity, 2013, 81 (3), pp.945-55. ⟨10.1128/IAI.00999-12⟩ |
| بيانات النشر: | HAL CCSD, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Models, Molecular, Time Factors, Protein Conformation, Mutant, MESH: Virulence, Legionella pneumophila, Mice, MESH: Protein Conformation, Gene expression, MESH: Animals, MESH: Bacterial Proteins, Regulation of gene expression, chemistry.chemical_classification, 0303 health sciences, MESH: Gene Expression Regulation, Bacterial, biology, Virulence, Amino acid, Infectious Diseases, Nicotinic agonist, Biochemistry, Female, MESH: Models, Molecular, Immunology, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Microbiology, Niacin, 03 medical and health sciences, Bacterial Proteins, MESH: Cell Proliferation, Animals, Gene, MESH: Mice, 030304 developmental biology, Cell Proliferation, Binding Sites, 030306 microbiology, Macrophages, MESH: Transcriptome, MESH: Time Factors, MESH: Macrophages, Gene Expression Regulation, Bacterial, biology.organism_classification, Molecular Pathogenesis, MESH: Legionella pneumophila, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, MESH: Niacin, chemistry, MESH: Binding Sites, Parasitology, NAD+ kinase, Lysosomes, Transcriptome, MESH: Female, MESH: Lysosomes |
| الوصف: | In response to environmental fluctuations or stresses, bacteria can activate transcriptional and phenotypic programs to coordinate an adaptive response. The intracellular pathogen Legionella pneumophila converts from a noninfectious replicative form to an infectious transmissive form when the bacterium encounters alterations in either amino acid concentrations or fatty acid biosynthesis. Here, we report that L. pneumophila differentiation is also triggered by nicotinic acid, a precursor of the central metabolite NAD + . In particular, when replicative L. pneumophila are treated with 5 mM nicotinic acid, the bacteria induce numerous transmissive-phase phenotypes, including motility, cytotoxicity toward macrophages, sodium sensitivity, and lysosome avoidance. Transcriptional profile analysis determined that nicotinic acid induces the expression of a panel of genes characteristic of transmissive-phase L. pneumophila . Moreover, an additional 213 genes specific to nicotinic acid treatment were altered. Although nearly 25% of these genes lack an assigned function, the gene most highly induced by nicotinic acid treatment encodes a putative major facilitator superfamily transporter, Lpg0273. Indeed, lpg0273 protects L. pneumophila from toxic concentrations of nicotinic acid as judged by analyzing the growth of the corresponding mutant. The broad utility of the nicotinic acid pathway to couple central metabolism and cell fate is underscored by this small metabolite's modulation of gene expression by diverse microbes, including Candida glabrata , Bordetella pertussis , Escherichia coli , and L. pneumophila . |
| اللغة: | English |
| تدمد: | 0019-9567 1098-5522 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0945bea75b895ef639ace6a1c919f94cTest https://hal-pasteur.archives-ouvertes.fr/pasteur-01422856/documentTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0945bea75b895ef639ace6a1c919f94c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00199567 10985522 |
|---|