دورية أكاديمية
Povećanje proizvodnje metabolita vitamina K2 dodatkom surfaktanta pri uzgoju mutanta bakterije Escherichia sp. FM3-1709 na tresilici ; Surfactant Supplementation to Enhance the Production of Vitamin K2 Metabolites in Shake Flask Cultures Using Escherichia sp. Mutant FM3-1709
| العنوان: | Povećanje proizvodnje metabolita vitamina K2 dodatkom surfaktanta pri uzgoju mutanta bakterije Escherichia sp. FM3-1709 na tresilici ; Surfactant Supplementation to Enhance the Production of Vitamin K2 Metabolites in Shake Flask Cultures Using Escherichia sp. Mutant FM3-1709 |
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| المؤلفون: | Liu, Yan, Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences, Anhui, 230031 Hefei, PR China, Zheng, Zhi-Ming, zmzheng@ipp.ac.cn, Qiu, Hong-Wei, National Center for Biotechnology Development, 100036 Beijing, PR China, Zhao, Gen-Hai, Wang, Peng, Liu, Hui, Wang, Li, Li, Zhe-Min, Wu, He-Fang, Liu, Hong-Xia, Tan, Mu |
| المصدر: | Food Technology and Biotechnology (ftb@pbf.hr); Vol.52 No.3; ISSN 1330-9862 (Print); ISSN 1334-2606 (Online) |
| بيانات النشر: | Faculty of Food Technology and Biotechnology, University of Zagreb; ftb@pbf.hr |
| سنة النشر: | 2014 |
| المجموعة: | Hrčak - Portal of scientific journals of Croatia / Portal znanstvenih časopisa Republike Hrvatske |
| مصطلحات موضوعية: | surfaktant, vitamin K2, uzgoj na tresilici, surfactant, shake flask cultures |
| الوصف: | Ispitan je utjecaj dodatka različitih surfaktanata na rast stanica i proizvodnju metabolita vitamina K2, poput unutarstaničnih i izvanstaničnih menakinona MK-4 i MK-6, pri submerznom uzgoju bakterije Escherichia sp. Svi su surfaktanti produljili eksponencijalnu fazu rasta bakterije. Betain, polioksietilen oleil eter i Tween-80 povoljno su djelovali na rast stanica mutanta bakterije Escherichia sp. FM3-1709. Najveći je rast stanica bio Xmax=(12,6±0,2) g/L, najveća stopa proizvodnje biomase Qx=(0,21±0,01) g/(L·h), a najveći prinos biomase YX/S=(2,42±0,02) g/g. Rezultati pokazuju da se dodatkom surfaktanata smanjila proizvodnja unutarstaničnog MK-4, dok se proizvodnja izvanstaničnog MK-4 bitno povećala. Od pet ispitanih surfaktanata, najbolji su rezultati postignuti dodatkom neionskog surfaktanta polioksietilen oleil etera (1,0 g/L), pri čemu je koncentracija izvanstaničnog MK-4 bila (33,6±0,4) mg/L, a izvanstaničnog MK-6 (2,56±0,07) mg/L, dok je ukupni prinos MK-4 bio (47,6±0,4) mg/L, a MK-6 (6,0±0,1) mg/L. Dodatak je polioksilen oleil etera djelovao puno povoljnije na lučenje MK-4, nego na lučenje MK-6. ; The effects of the addition of various surfactants on the cell growth and production of vitamin K2 metabolites, such as intracellular menaquinone-4 (MK-4), extracellular MK-4, intracellular MK-6 and extracellular MK-6, were studied in the submerged culture of Escherichia sp. All of the added surfactants caused the extension of the exponential phase. Betaine, polyoxyethylene oleyl ether and Tween-80 were favourable to the cell growth of Escherichia sp. mutant strain FM3-1709. The highest cell growth (Xmax), biomass production rate (Qx) and biomass yield (Yx/s) were (12.6±0.2) g/L, (0.21±0.01) g/(L·h) and (2.42± 0.02) g/g, respectively. The results show that the addition of all surfactants led to a lower production of intracellular MK-4, whereas the production of extracellular MK-4 increased remarkably. Among the five investigated surfactants, the addition of the nonionic surfactant polyoxyethylene oleyl ether ... |
| نوع الوثيقة: | text |
| وصف الملف: | |
| اللغة: | Croatian English |
| العلاقة: | http://hrcak.srce.hr/126170Test; http://hrcak.srce.hr/file/186419Test; http://hrcak.srce.hr/file/186420Test |
| الإتاحة: | http://hrcak.srce.hr/126170Test http://hrcak.srce.hr/file/186419Test http://hrcak.srce.hr/file/186420Test |
| حقوق: | The contents of the Food Technology and Biotechnology can be used for personal, educational or scientific purposes, provided that the credit is given to the Journal (under Creative Commons licence CC BY-NC 4.0). Annual subscription price for all countries (except Croatia) is 40 euro for citizens and 100 euro for organizations and institutions. Discount is offered to the members of Croatian Society of Biotechnology and Slovenian Microbiological Society. For subscription please contact the Editorial Office at the E-mail address: ftb@pbf.hr |
| رقم الانضمام: | edsbas.51D7F92F |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |