Contribution of the TCRβ Repertoire to Marek’s Disease Genetic Resistance in the Chicken
| العنوان: | Contribution of the TCRβ Repertoire to Marek’s Disease Genetic Resistance in the Chicken |
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| المؤلفون: | Hearn, Cari J., Cheng, Hans H. |
| المصدر: | Viruses Volume 15 Issue 3 Pages: 607 |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute, 2023. |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | TCR repertoire, genetic resistance, poultry immunogenetics, Marek’s disease virus |
| الوصف: | Marek’s disease (MD) is a lymphoproliferative disease of chickens induced by Marek’s disease virus (MDV), an oncogenic α-herpesvirus. MDV has increased in virulence, prompting continued efforts in both improved vaccines and enhanced genetic resistance. Model pairs of genetically MD-resistant and MD-susceptible chickens that were either MHC-matched or MHC-congenic allowed characterization of T cell receptor (TCR) repertoires associated with MDV infection. MD-resistant chickens showed higher usage of Vβ-1 TCRs than susceptible chickens in both the CD8 and CD4 subsets in the MHC-matched model, and in the CD8 subset only in the MHC-congenic model, with a shift towards Vβ-1+ CD8 cells during MDV infection. Long and short read sequencing identified divergent TCRβ loci between MHC-matched MD-resistant and MD-susceptible chickens, with MD-resistant chickens having more TCR Vβ1 genes. TCR Vβ1 CDR1 haplotype usage in MD-resistant x MD-susceptible F1 birds by RNAseq indicated that the most commonly used CDR1 variant was unique to the MD-susceptible line, suggesting that selection for MD resistance in the MHC-matched model optimized the TCR repertoire away from dominant recognition of one or more B2 haplotype MHC molecules. Finally, TCR downregulation during MDV infection in the MHC-matched model was strongest in the MD-susceptible line, and MDV reactivation downregulated TCR expression in a tumor cell line. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1999-4915 |
| DOI: | 10.3390/v15030607 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=multidiscipl::d58edd8c671cc35b175880ce997afc6eTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.multidiscipl..d58edd8c671cc35b175880ce997afc6e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19994915 |
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| DOI: | 10.3390/v15030607 |