The Arabidopsis F-box protein FBW2 targets AGO1 for degradation to prevent spurious loading of illegitimate small RNA
| العنوان: | The Arabidopsis F-box protein FBW2 targets AGO1 for degradation to prevent spurious loading of illegitimate small RNA |
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| المؤلفون: | Thibaut Hacquard, Marion Clavel, Patricia Baldrich, Esther Lechner, Imma Pérez-Salamó, Mikhail Schepetilnikov, Benoît Derrien, Marieke Dubois, Philippe Hammann, Lauriane Kuhn, Danaé Brun, Nathalie Bouteiller, Nicolas Baumberger, Hervé Vaucheret, Blake C. Meyers, Pascal Genschik |
| المساهمون: | Institut de biologie moléculaire des plantes (IBMP), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Donald Danforth Plant Science Center, Plateforme Protéomique Strasbourg Esplanade du CNRS, Universite ́ de Strasbourg, 67084 Strasbourg, France, Institut Jean-Pierre Bourgin (IJPB), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), The mass spectrometry instrumentation was funded by the University of Strasbourg, IdEx ‘‘Equipement mi-lourd’’ 2015. Work in the Meyers lab was supported by US National Science Foundation IOS award 1842685 and resources from the Donald Danforth Plant Science Center and the University of Missouri., ANR-10-LABX-0036,NetRNA,Network of regulatory RNAs across kingdoms and dynamical responses to biotic and abiotic stresses.(2010), ANR-10-IDEX-0002,UNISTRA,Par-delà les frontières, l'Université de Strasbourg(2010), ANR-17-EURE-0023,IMCBio,Integrative Molecular and Cellular Biology(2017), European Project: 338904,EC:FP7:ERC,ERC-2013-ADG,PHAGORISC(2014) |
| المصدر: | Cell Reports Cell Reports, 2022, 39 (2), pp.110671. ⟨10.1016/j.celrep.2022.110671⟩ |
| بيانات النشر: | Elsevier BV, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | CP: plants, Arabidopsis Proteins, [SDV]Life Sciences [q-bio], F-Box Proteins, Arabidopsis, Arbidopsis, proteolysis, RNA cleavage, General Biochemistry, Genetics and Molecular Biology, MicroRNAs, CP: molecular biology, Argonaute Proteins, RNA Interference, RNA, Small Interfering, RNA silencing |
| الوصف: | International audience; RNA silencing is a conserved mechanism in eukaryotes involved in development and defense against viruses. In plants, ARGONAUTE1 (AGO1) protein plays a central role in both microRNA- and small interfering RNA- directed silencing, and its expression is regulated at multiple levels. Here, we report that the F-box protein FBW2 assembles an SCF complex that selectively targets for proteolysis AGO1 when it is unloaded and mutated. Although FBW2 loss of function does not lead to strong growth or developmental defects, it signif- icantly increases RNA-silencing activity. Interestingly, under conditions in which small-RNA accumulation is affected, the failure to degrade AGO1 in fbw2 mutants becomes more deleterious for the plant. Accordingly, the non-degradable AGO1 protein assembles high-molecular-weight complexes and binds illegitimate small RNA, leading to off-target cleavage. Therefore, control of AGO1 homeostasis by FBW2 plays an important role in quality control of RNA silencing. |
| تدمد: | 2211-1247 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b5eaf9c33e998723f005c95329d96054Test https://doi.org/10.1016/j.celrep.2022.110671Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b5eaf9c33e998723f005c95329d96054 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22111247 |
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