Retrofitting baculoviral vector with Sleeping Beauty transposon system: competent for long-term reporter gene imaging in vivo
| العنوان: | Retrofitting baculoviral vector with Sleeping Beauty transposon system: competent for long-term reporter gene imaging in vivo |
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| المؤلفون: | Jinxin Zhou, Min Yang, Jing Lv, Yifan Zhang, Donghui Pan, Liying Zhu, Yu Pan, Huijun Ju |
| المصدر: | Applied Microbiology and Biotechnology. 102:1933-1943 |
| بيانات النشر: | Springer Science and Business Media LLC, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Transposable element, Time Factors, Carcinogenesis, Transgene, Green Fluorescent Proteins, Gene Expression, Mice, Nude, Biology, Applied Microbiology and Biotechnology, Glucagon-Like Peptide-1 Receptor, Green fluorescent protein, 03 medical and health sciences, Genes, Reporter, In vivo, Neoplasms, Animals, Recombination, Genetic, Reporter gene, Staining and Labeling, Chimera, Optical Imaging, General Medicine, Sleeping Beauty transposon system, Cell biology, Transplantation, Disease Models, Animal, 030104 developmental biology, Positron-Emission Tomography, DNA Transposable Elements, Molecular imaging, Baculoviridae, Biotechnology |
| الوصف: | Reporter gene imaging is widely used for non-invasively detecting tumorigenesis, trafficking therapeutic cells, and monitoring treatment effect. Baculoviral vectors (BVs) have been utilized as transgenic vectors in the reporter gene imaging systems in recent years. However, BV-mediated report gene imaging can only provide short-term investigation due to its transient transgene expression, which is incompetent for the long-term applications. In the current study, we reconstructed a series of hybrid BVs with several elements, to investigate the feasibility of this hybrid BV-mediated long-term reporter gene imaging in vivo. We showed that with the indispensable assistance of a positive-selection process, hybrid BV containing Sleeping Beauty 100× (SB) transposon system (BV-SB) could significantly prolong the enhanced green fluorescent protein (eGFP) expression for at least 180 days in vitro at nearly 100% eGFP positive percentage and over 1011 arbitrary unit total fluorescence intensity, whereas other hybrid BV-mediated transgene expression gradually faded in only 20 days. Furthermore, BV-SB-mediated eGFP fluorescent reporter gene imaging monitored tumorigenesis in the nude mice for at least 35 days. In addition, we exploited the glucagon-like peptide 1 receptor (glp-1r) gene as a radionuclide reporter gene for in vivo micro-PET imaging. At 50th day post-tumor transplantation, the micro-PET imaging showed considerable radiotracer-receptor-binding in vivo, resulted by stable high level of BV-SB-mediated GLP-1R expression in tumor. In summary, we retrofitted BV with the SB transposon system to make it competent for the long-term reporter gene imaging in vivo, which might broaden the application scopes of BV in the long-term molecular imaging and other biomedicine research fields. |
| تدمد: | 1432-0614 0175-7598 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0d6d856037a71f10e81de9b3a2a3f807Test https://doi.org/10.1007/s00253-018-8780-4Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....0d6d856037a71f10e81de9b3a2a3f807 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14320614 01757598 |
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