دورية أكاديمية
Human pancreatic islet endothelial cells express coxsackievirus and adenovirus receptor and are activated by coxsackie B virus infection
| العنوان: | Human pancreatic islet endothelial cells express coxsackievirus and adenovirus receptor and are activated by coxsackie B virus infection |
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| المؤلفون: | M. M. ZANONE, E. FAVARO, G. C. HUANG, N. J. KLEIN, P. CAVALLO PERIN, M. PEAKMAN, G. CAMUSSI, FERIOLI, ELENA, CONALDI, PIER GIULIO |
| المساهمون: | M. M., Zanone, E., Favaro, Ferioli, Elena, G. C., Huang, N. J., Klein, P., CAVALLO PERIN, M., Peakman, Conaldi, PIER GIULIO, G., Camussi |
| سنة النشر: | 2007 |
| المجموعة: | IRInSubria - Institutional Repository Insubria (Università degli Studi dell’Insubria) |
| مصطلحات موضوعية: | autoimmunity, adhesion molecule, cytokine, viral infection |
| الوصف: | Enteroviruses, such as the coxsackievirus (CV) group, have been linked to the induction of inflammatory and autoimmune diseases. Virus tropism and tissue access are modulated by endothelial cells. To examine the susceptibility of microvascular endothelial cells (MECs) derived from pancreatic islets to infection with CV group B (CVB), purified cultured human islet MECs were infected with CVB-4 strain, and the immunological phenotype of the infected cells was analyzed. CVB-4 persistently infected the islet MECs, which expressed the CV receptors human coxsackievirus and adenovirus receptor (HCAR) and decay accelerating factor (DAF) and maintained EC characteristics, without overt cytopathic effects. CVB-4 infection transiently up-regulated expression of the adhesion molecules ICAM-1 and VCAM-1 and increased production of the proinflammatory cytokines IL-1ß and IL-6, and chemokines IL-8 and lymphotactin, as well as IFN-. Mononuclear cell adhesion to CVB infected monolayers was increased, compared to uninfected monolayers. Moreover, infection up-regulated the viral receptors HCAR and DAF and coreceptor vß3 integrin on islet MECs, while down-regulating expression of HCAR on human aortic endothelial cells, indicating potential tissue-specific influence on the pathological outcome of infection. These results provide evidence that islet MECs are natural targets and reservoirs for persistent CVB infection resulting in acute endothelial cell activation by virus, which may contribute to selective recruitment of subsets of leukocytes during inflammatory immune responses, such as insulitis in type 1 diabetes. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| العلاقة: | info:eu-repo/semantics/altIdentifier/wos/WOS:000249781600031; volume:21; firstpage:3308; lastpage:3317; numberofpages:10; journal:THE FASEB JOURNAL; http://hdl.handle.net/11383/1683426Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-35248864495 |
| DOI: | 10.1096/fj.06-7905com |
| الإتاحة: | https://doi.org/10.1096/fj.06-7905comTest http://hdl.handle.net/11383/1683426Test |
| رقم الانضمام: | edsbas.1483F40C |
| قاعدة البيانات: | BASE |
| DOI: | 10.1096/fj.06-7905com |
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