دورية أكاديمية
Isolation, identification and molecular characterization of Brucella strains isolated from small ruminants in the West Bank, Palestine
| العنوان: | Isolation, identification and molecular characterization of Brucella strains isolated from small ruminants in the West Bank, Palestine |
|---|---|
| المؤلفون: | Awwad, Elena, Farraj, Mohammad, Adwan, Kamel M., Essawi, Tamer |
| بيانات النشر: | Revue de Medecine Veterinaire |
| سنة النشر: | 2011 |
| المجموعة: | FADA - Birzeit University Open Access Repository |
| مصطلحات موضوعية: | Brucellosis - Palestine, Small ruminants - Palestine, Serological test - Palestine, B. melitensis - Palestine, Bacterial isolation - Palestine, PCR assay - Palestine, Phylogenic analysis - Palestine |
| الوصف: | The seroprevalence of brucellosis in the small ruminant (sheep and goat) population from infected flocks was investigated in the West Bank (Palestine). 36.8% (129/350) and 32.3% (113/350) of animals were found to be positive by the Rose Bengal test (RBT) and the complement fixation test (CFT) respectively, the agreement score between the 2 methods being 89.1%. The presence of Brucella directly assessed by bacterial isolation and PCR assay in milk samples from 80 seropositive animals was confirmed in 47.5% (38/80) and in 77.5% (62/80) of samples, leading to a moderate agreement score between the 2 methods (70.0%). The phylogenic analysis of a putative open reading frame (ORF) in the haemagglutinin gene revealed that the current B. melitensis isolates were genetically close to those from different Mediterranean countries (France, Spain and Cyprus) and particularly to the Israel strain. This first report of DNA sequencing of Brucella strains in small ruminants in Palestine would greatly help to implement control and preventive measures for brucellosis. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | http://hdl.handle.net/20.500.11889/7649Test |
| الإتاحة: | https://doi.org/20.500.11889/7649Test https://hdl.handle.net/20.500.11889/7649Test |
| رقم الانضمام: | edsbas.B883E3B1 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |